Tambopata National Reserve (Spanish: Reserva Nacional Tambopata) is a nature reserve in the Peruvian Amazon Basin south of the Madre de Dios River in Tambopata Province's Inambari and Tambopata districts. It was created on January 26, 1990, to protect the forests adjacent to the rivers Heath and Tambopata that have two important ecosystems and are noted for its biodiversity, representing native flora and fauna with over 1700 species (comprising 127 families and 622 genera) of plants, 103 species of mammals, 1300 species of butterflies and 90 species of amphibians. The reserve is located across the regions of Madre de Dios and Puno: Tambopata is the 40% of the reserve in the department of Madre de Dios. Carabaya and Sandia are the 60% of the reserve in the department of Puno. Access is from Puerto Maldonado, where one can get to the preserve via the Tambopata River; or by car via the Puerto Maldonado–Cuzco Road (kilometer 30).